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GOOD LABORATORY PRACTICE WHEN PERFORMING MOLECULAR AMPLIFICATION ASSAYS

STATUS OF NATIONAL STANDARD METHODS
National Standard Methods, which include standard operating procedures (SOPs), algorithms and guidance notes, promote high quality practices and help to assure the comparability of diagnostic information obtained in different laboratories. This in turn facilitates standardisation of surveillance underpinned by research, development and audit and promotes public health and patient confidence in their healthcare services. The methods are well referenced and represent a good minimum standard for clinical and public health microbiology. However, in using National Standard Methods, laboratories should take account of local requirements and may need to undertake additional investigations. The methods also provide a reference point for method development.

National Standard Methods are developed, reviewed and updated through an open and wide consultation process where the views of all participants are considered and the resulting documents reflect the majority agreement of contributors.

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Nucleic Acids Calculator Enter Parameters OD260 OD280 Dilution factor DNA/RNA Stock Volume (ul) type of nucleic acid   select  dsDNA  RNA  ssDNA  ssOligo      Results measured DNA/RNA conc (ug/ ul) OD260/OD280 ratio stock DNA/RNA conc (ug/ ul) Total Amount of DNA/RNA ( ug) 1 OD260 Unit = 50ug/ml for double-stranded DNA 1 OD260 Unit = 40ug/ml for single-stranded RNA 1 OD260 Unit = 40ug/ml for single-stranded DNA 1 OD260 Unit = 20ug/ml for single-stranded oligonucleotides http://www.kenkyuu.net/js/nacalc.html