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Methods for detection of GMOs in food and feed

Methods for detection of GMOs in food and feed
Nelson Marmiroli & Elena Maestri & Mariolina Gullì & Alessio Malcevschi & Clelia Peano & Roberta Bordoni & Gianluca De Bellis
Received: 30 April 2008 /Revised: 14 July 2008 /Accepted: 17 July 2008 / Published online: 23 August 2008 © Springer-Verlag 2008

Abstract This paper reviews aspects relevant to detection and quantification of genetically modified (GM) material within the feed/food chain. The GM crop regulatory framework at the international level is evaluated with reference to traceability and labelling. Current analytical methods for the detection, identification, and quantification of transgenic DNA in food and feed are reviewed. These methods include quantitative real-time PCR, multiplex PCR, and multiplex real-time PCR. Particular attention is paid to methods able to identify multiple GM events in a single reaction and to the development of microdevices and microsensors, though they have not been fully validated for application.

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