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Methods for detection of GMOs in food and feed Nelson Marmiroli & Elena Maestri & Mariolina Gullì & Alessio Malcevschi & Clelia Peano & Roberta Bordoni & Gianluca De Bellis Received: 30 April 2008 /Revised: 14 July 2008 /Accepted: 17 July 2008 / Published online: 23 August 2008 © Springer-Verlag 2008 Abstract This paper reviews aspects relevant to detection and quantification of genetically modified (GM) material within the feed/food chain. The GM crop regulatory framework at the international level is evaluated with reference to traceability and labelling. Current analytical methods for the detection, identification, and quantification of transgenic DNA in food and feed are reviewed. These methods include quantitative real-time PCR, multiplex PCR, and multiplex real-time PCR. Particular attention is paid to methods able to identify multiple GM events in a single reaction and to the development of microdevices and microsensors, though they have not been...

STATUS OF NATIONAL STANDARD METHODS National Standard Methods, which include standard operating procedures (SOPs), algorithms and guidance notes, promote high quality practices and help to assure the comparability of diagnostic information obtained in different laboratories. This in turn facilitates standardisation of surveillance underpinned by research, development and audit and promotes public health and patient confidence in their healthcare services. The methods are well referenced and represent a good minimum standard for clinical and public health microbiology. However, in using National Standard Methods, laboratories should take account of local requirements and may need to undertake additional investigations. The methods also provide a reference point for method development. National Standard Methods are developed, reviewed and updated through an open and wide consultation process where the views of all participants are considered and the resulting documents reflect the...

HANDBOOK GOOD LABORATORY PRACTICE (GLP) Quality practices for regulated non-clinical research and development FOREWORD In order to assist countries in conducting non-clinical research and drug development, TDR developed a Good Laboratory Practices (GLP) series in 2001, comprising a GLP Handbook as well as GLP Training manuals for trainers and trainees. The demand for this series was so substantial that it became one of the most frequent “hits” on the TDR website, generating interest and demand for a second edition. This Second-edition GLP series is presented here in a revised and updated format. It supports continued tech- nology transfer and capacity-building in disease endemic countries (DECs) in line with the aims of the recent World Health Assembly Resolution (WHA 61.21) on a Global strategy and plan of action on public health, innovation and intellectual property. https://docs.google.com/document/d/1fyEmcuQHdzLKTUZypAn-vLTAzpNIzdz_SHVhDnUQwXo/edit

GLP: Good Laboratory Practice 1. Introduction In the early 70’s FDA (United States Food and Drug administration) have realized cases of poor laboratory practice throughout the United States. FDA decided to check over 40 toxicology labs in-depth. They revealed lot dishonest activities and a lot of poor lab practices. Examples of some of these poor lab practices found were equipment not been calibrated to standard form, therefore giving wrong measurements, incorrect or inaccurate accounts of the actual lab study and incompetent test systems. Although the term “good laboratory practice” might have been used informal already for some time in many laboratories around the world GLP originated in the United States and it had a powerfull effect world wide. 2. History of Good Laboratory Practice (GLP) GLP is an official regulation that was created by the FDA in 1978. The OECD (Organisation for Economic Co-operation and Development) Principles of Good Laboratory Practice were first cr...

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Abstract Standards for laboratory investigations; GLP principles are defined by the EC (I) as: “principles of good laboratory practice, that are consistent with the OECD principles of good laboratory practice as adopted in article 1 of directive 87/18/EEC”. GLP: Good Laboratory Practice Isin Akyar Acibadem University Faculty of Medicine D epartment of Medical Microbiology Turkey 1. Introduction In the early 70’s FDA (United States Food an d Drug administration) have realized cas es of poor laboratory practice throughout the Un ited States. FDA decided to check over 40 toxicology labs in-depth. They revealed lot dishonest activities and a lot of poor lab practices. Examples of some of these poor lab practices found were equipment not been calibrated to standard form, th erefore giving wrong measuremen ts, incorrect or inaccurate accounts of the actual lab study and incompet ent test systems. Alth ough the term “good laboratory practice” might have been u...